CristalO - crystallography core facility
Our platform’s core mission is to support, monitor, and oversee projects that rely on protein-applied crystallography. All our services are offered within the EMBRC-France infrastructure.
Crystallogenesis and Structure Determination
The crystallogenesis service involves producing crystals from a soluble protein. Crystallization conditions are automatically identified using a crystallization robot before the manual steps of reproducing and optimizing the crystals.
Structure determination is then performed using protein crystallography, which consists of exposing the crystals to synchrotron X-rays to obtain diffraction, collecting the data, and carrying out the resolution and refinement of the 3D coordinates.
Infrastructure de recherche
Contact(s)
- Alexandra Jeudy
Services
We make our expertise available to provide you with the following services:
- Determination of the oligomeric state of a sample by DLS
- Sample crystallization using a Crystal Gryphon crystallization robot
- Monitoring of crystallization with a CrysCam camera and/or a stereomicroscope
- Manual reproduction and optimization of crystallization conditions
- Determination of cryo-freezing conditions for crystals
- X-ray diffraction of crystals
- Data collection and processing
- Phasing, reconstruction, and refinement of the 3D structure
Our expertise focuses on biomolecules, namely:
• Soluble proteins
• Protein complexes including hydrophilic and hydrophobic molecules
• Protein–protein complexes
• Protein–nucleic acid complexes
Team
