Chargé(e) de recherche CNRS

email: cherve@sb-roscoff.fr

EXTRACELLULAR MATRICES IN BROWN ALGAE

MY RESEARCH PROJECT

My research interests are focused on the extracellular matrices (ECMs) or cell walls of marine macroalgae, in relation to their composition, biological functions and biosynthesis. Brown algae are my main current models. I aim to gain an exhaustive view of the components of the ECM and to explore their interactions. I study their roles in relation to different physiological responses like embryogenesis, early development or salinity variations. I explore their corresponding biosynthetic pathways through the study of candidate genes and their functional validation by the characterisation of native and/or recombinant enzymes.

 

I use a variety of approaches with: the extraction and purification of glycans, imaging studies combined with immunohistochemistry techniques using dedicated in-house antibodies, high throughput techniques for profiling, as well as organelle isolation and heterologous expression of proteins in prokaryotic and eukaryotic systems.

 

 

I am coordinating a collaborative project funded by the French ANR agency and entitled BrownSugar (2020-2024). We aim to provide a better understanding of the biosynthesis of the extracellular matrix in brown algae by examining corresponding glycosyltransferases, from gene identification to protein expression, purification and biochemical characterization.

 

 

SERVICES

We maintain the cell lines producing the Brown Algal Monoclonal (BAM) antibodies against sulfated fucans (BAM1-4) and alginates (BAM6-11).
Our monoclonal antibodies are available for use through SeaProbes.

 

PEOPLE

Ahlem Bouguerba (PhD student)
Lisa Mazéas (PhD student)
Murielle Jam (lab technician)

former -Delphine Duffieux (lab technician)
former -Amandine Siméon (PhD student)
former -Kevin Hardouin (post-doctoral fellow)
former -Sonia Kridi (lab technician)

last update: 12/10/2021

 

 

Publications

A SELECTION IS SHOWN

For a more detailed list, see ORCID: 0000-0001-6649-8137

Yonamine R, Ichihara K, Tsuyuzaki S, Hervé C, Motomura T,  Nagasato C (2021). Changes in Cell Wall Structure During Rhizoid Formation of Silvetia babingtonii (Fucales, Phaeophyceae) zygotes. Journal of Phycology, 57(4), 1356-1367.

Dittami SM, Corre E, Brillet-Guéguen L, Lipinska AP, Pontoizeau N, Aite M, Avia K, Caron C, Cho CH, Collén J, Cormier A, Delage L, Doubleau S, Frioux C, Gobet A, González-Navarrete I, Groisillier A, Hervé C, Jollivet D, KleinJan H, Leblanc C, Liu X, Marie D, Markov GV, Minoche AE, Monsoor M, Pericard P, Perrineau M-M, Peters AF, Siegel A, Siméon A, Trottier C, Yoon HS, Himmelbauer H, Boyen C, Tonon T (2020). The genome of Ectocarpus subulatus – A highly stress-tolerant brown alga. Marine Genomics, 100740.

Salmeán AA, Guillouzo A, Duffieux D, Jam M, Matard-Mann M, Larocque R, Pedersen HL, Michel G, Czjzek M, Willats WGT, Hervé C (2018). Double blind microarray-based polysaccharide profiling enables parallel identification of uncharacterized polysaccharides and carbohydrate-binding proteins with unknown specificities. Scientific Reports 8, 2500.

Deniaud-Bouët E, Hardouin K, Potin P, Kloareg B, Hervé C (2017). A review about brown algal cell walls and fucose-containing sulfated polysaccharides: Cell wall context, biomedical properties and key research challenges. Carbohydrate polymers 175, 395-408.

Siméon A, Hervé C (2017). Isolation of Fucus serratus Gametes and Cultivation of the Zygotes. Bio-protocol 7(14): e2408.

Salmeán AA, Duffieux D, Harholt J, Qin F, Michel G, Czjzek M, Willats WGT, Hervé C (2017). Insoluble (1 → 3), (1 → 4)-β-D-glucan is a component of cell walls in brown algae (Phaeophyceae) and is masked by alginates in tissues. Scientific Reports, 7(1), 2880

Torode AT, Siméon A, Marcus SE, Jam M, Le Moigne M-A, Duffieux D, Knox JP, Hervé C (2016). Dynamics of cell wall assembly during early embryogenesis in the brown alga Fucus. Journal of Experimental Botany. 61(21): 6089-6100

Ropartz D, Giuliani A, Fanuel M, Hervé C, Czjzek M, Rogniaux H (2016). Online Coupling of High-Resolution Chromatography with Extreme Uv Photon Activation Tandem Mass Spectrometry: Application to the Structural Investigation of Complex Glycans by Dissociative Photoionization. Analytica Chimica Acta. 933: 1-9

Fischl R, Bertelsen K, Gaillard F, Coelho S, Michel G, Klinger M, Boyen C, Czjzek M, Hervé C (2016). The cell wall active mannuronan C5-epimerases in the model brown alga Ectocarpus: from gene context to recombinant protein. Glycobiology. 26(9): 973-983

Hervé C, Siméon A, Jam M, Cassin A, Johnson KL, Salmeán AA, Willats WG, Doblin MS, Bacic A, Kloareg B (2016). Arabinogalactan proteins have deep roots in eukaryotes: identification of genes and epitopes in brown algae and their role in Fucus serratus embryo development. New Phytologist. 209: 1428–1441

Ficko-Blean E, Hervé C, Michel G (2015). Sulfated sugars from the sea: biosynthesis and remodelling of sulfated macroalgal cell wall polysaccharides. Perspectives in Phycology 2(1): 51-64.

Torode TA, Marcus SE, Jam M, Tonon T, Blackburn RS, Hervé C, Knox JP (2015). Monoclonal antibodies directed to fucoidan preparations from brown algae. PLoS One 10(2): e0118366.

Ropartz D, Giuliani A, Hervé C, Geairon A, Jam M, Czjzek C, Rogniaux H (2015). High-Energy Photon Activation Tandem Mass Spectrometry Provides Unprecedented Insights into the Structure of Highly Sulfated Oligosaccharides Extracted from Macroalgal Cell Walls. Analytical Chemistry 87(2): 1042-49.

Tsirigoti A, Beakes G, Hervé C, Gachon CM, Katsaros C (2014) Attachment, penetration and early host defense mechanisms during the infection of filamentous brown algae by Eurychasma dicksonii. Protoplasma: 1-12.

Deniaud-Bouët E, Kervarec N, Michel G, Tonon T, Kloareg B, Hervé C (2014) Chemical and enzymatic fractionation of cell walls from Fucales: insights into the structure of the extracellular matrix of brown algae. Annals of Botany 114: 1203-1216.

Popper ZA, Michel G, Hervé C, Domozych D, Willats WGT, Tuohy MG, Kloareg B, Stengel DB (2011). Evolution and Diversity of Plant Cell Walls: From Algae to Flowering Plants. Annu Rev Plant Biol. 62: 567-590.