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Co-responsable Equipe DYDIV

Thomas Broquet


1- New project: reproductive barriers and speciation


  • I recently initiated a project focusing on speciation in a complex of interfertile species. Focusing on the Jaera albifrons group (marine isopods) this project aims to analyze the processes that prevent gene flow across species.
  • We focus here on two such isolating barriers that were shown to be decisive for the diversification of animals. The first one is behavioral isolation, which results from mate choice preferences for conspecifics during sexual reproduction. One pertaining issue in this context is the link between such mate choice processes between species with sexual selection processes occurring within species. The second isolating barrier that we will be looking at is postzygotic genetic incompatibilities.


Jaera albifrons


Sampling in Normandy



2- Life-cycle, evolutionary forces, and genetic variation in marine invertebrates


  • I'm interested in understanding how life-history traits (primarily dispersal and reproductive system) control the dynamics of neutral genetic variation within species. 
  • My current research along these lines deals with the genetic structure of marine invertebrates, where results from previous studies have suggested that stochastic effects during reproduction (and thus genetic drift) interact with complex patterns of dispersal (and thus gene flow) to create chaotic patterns of genetic variation. I use analytical and simulation-based approaches to formalize and explore the role of genetic drift and gene flow in generating chaotic patterns of genetic structure as observed in marine invertebrates.
  • I also use the gastropod Crepidula fornicata (slipper limpet) in empirical studies for analyzing the causes and consequences of genetic drift in a marine benthic invertebrate. This work led to an empirical evaluation of the size-advantage hypothesis of sex-allocation in protandrous species.


Slipper limpets (Crepidula fornicata)



3- Collaborations


  • Genetic study of dispersal and demography of the Ivory gull Pagophila eburnea (collab. Glenn Yannic, Emmanuelle Yannic, the Groupe de Recherches en Ecologie Arctique (GREA), and ivory gull researchers worldwide)




  • Castel J, Pradillon F, Cueff V, Leger G, Daguin-Thiébaut C, Ruault S, Mary J, Hourdez S, Jollivet D and Broquet T (preprint). Genetic sex determination in three closely related hydrothermal vent gastropods, one of which has intersex individuals. doi: 10.1101/2023.04.11.536409

  • Mouchi V, Pecheyran C, Claverie F, Cathalot C, Matabos M, Rouxel O, Jollivet D, Broquet T, and Comtet T (preprint). A step towards measuring connectivity in the deep-sea: elemental fingerprints of mollusk larval shells discriminate hydrothermal sites.  doi: 10.1101/2023.01.03.522618v1

  • Broquet T, Destombe C, Valéro M, and Jollivet D (2023). A brief history of speciation research at the Station Biologique de Roscoff (1872-2022). Cahiers de Biologie Marine 64:9-19 (special issue 150 years anniversary of the station). doi: 10.21411/CBM.A.3DAF598C

  • Stankowski S, Zagrodzka ZB, Galindo J, Montaño-Rendón M, Faria R, Mikhailova N, Blakeslee AMH, Arnason E, Broquet T, Morales HE, Grahame JW, Westram AM, Johannesson K and Butlin RK (2023). Whole-genome phylogeography of the intertidal snail Littorina saxatilis. Evolutionary Journal of the Linnean Society. 2: kzad002. doi: 10.1093/evolinnean/kzad002

  • Yannic G, Hoste A, Capblancq T, Broquet T, Denoyelle L, Perrier C, Buzan E V, Šprem N, Corlatti L, Crestanello B, Hauffe H, and Pellissier L (in press). Projection of current and future distribution of adaptive genetics unite in an alpine ungulate. Heredity.

  • Gousy-Leblanc M, Therrien J-F, Broquet T, Rioux D, Curt-Grand-Gaudin N, Tissot N, Tissot S, Szabo I, Wilson L, Evans J. T., Bowes V, Gauthier G, Wiebe K. L., Yannic G and Lecomte N (2023). Long-term population decline of a genetically homogenous continental-wide top Arctic predator. Ibis 165:1251-1266. doi: 10.1111/ibi.13199

  • Mouchi V, Broquet T, and Comtet T (2022). Preparation of mollusc larval shells for individual geochemical analysis.

  • Castel J, Hourdez S, Pradillon F, Daguin-Thiébaut C, Ballenghien M, Ruault S, Corre E, Tran Lu Y A, Mary J, Gagnaire P-A, Bonhomme F, Breusing C, Broquet T and Jollivet D (2022). Inter-specific genetic exchange despite strong divergence in deep-sea hydrothermal vent gastropods of the genus Alviniconcha. Genes 13(6): 985. [pdf]

  • Breusing C, Castel J, Yang Y, Broquet T, Sun J, Jollivet D, Qian P-Y, Beinart RA (2022). Global 16S rRNA diversity of provannid snail endosymbionts from Indo-Pacific deep-sea hydrothermal vents. Environmental Microbiology Reports 14(2): 299-307. [pdf]

  • Tran Lu Y A, Ruault S, Daguin-Thiébaut C, Castel J, Bierne N, Broquet T, Wincker P, Perdereau A, Arnaud-Haond S, Gagnaire P-A, Jollivet D, Hourdez S, Bonhomme F (2022). Subtle limits to connectivity revealed by outlier loci within two divergent metapopulations of deep-sea hydrothermal gastropod Ifremeria nautilei. Molecular Ecology 31(10): 2796-2813. [pdf]

  • Boulart C, Rouxel O, Scalabrin C, Le Meur P, Pelleter E, Poitrimol C, Thiébaut E, Matabos M, Castel J, Tran Lu Y A, Michel LN, Cathalot C, Chéron S, Boissier A, Germain Y, Guyader V, Arnaud-Haond S, Bonhomme F, Broquet T, Cueff-Gauchard V, Le Layec V, L'Haridon S, Mary J,  Le Port A-S, Tasiemski A, Kuama DC, Hourdez S, Jollivet 
D (2022). Active hydrothermal vents in the Woodlark Basin may act as dispersing centres for hydrothermal fauna. Communications Earth & Environment 3: 64.

  • Leugger F, Broquet T, Karger DK, Rioux D, Buzan E, Corlatti L, Crestanello B, Curt-Grand-Gaudin N, Hauffe HC, Rolečková B, Šprem N, Tissot N, Tissot S, Yannic G, Pellissier L. (2022). Dispersal and habitat dynamics shape the genetic structure of the Northern chamois in the Alps. Journal of Biogeography 49: 1848-1861.

  • Charbonnel E, Daguin-Thiébaut C, Caradec  L, Moittié E, Gilg O, Gavrilo MV, Strøm H, Mallory ML, Morrison RIG, Gilchrist HG, Leblois R, Roux C, Yearsley JM, Yannic G, Broquet 
T (2022). Searching for genetic evidence of demographic decline in a 
long-lived seabird: beware of overlapping generations. Heredity 128: 364-376.

  • Barry P, Broquet T, Gagnaire P-A (2022). Age-specific survivorship and fecundity shape genetic diversity in marine fishes. Evolution Letters 6: 46-62.

  • Ribardière A,  Pabion E, Coudret J, Daguin-Thiébaut C, Houbin C, Loisel S, Henry S, and Broquet T (2021). Sexual isolation with and without ecological isolation in marine isopods Jaera albifrons and J. praehirsuta. Journal of Evolutionary Biology 34: 33-48[pdf]

  • Daguin-Thiébaut C, Ruault S, Roby C, Broquet T, Viard F, Brelsford A (2021). Construction of individual ddRAD libraries.

  • Stankowski S, Westram A, Zagrodzka Z B, Eyres I, Broquet T, Johannesson K and Butlin R. K. (2020). The evolution of strong reproductive isolation between sympatric intertidal snails. Philosophical transactions of the Royal Society of London 375: 20190545

  • Ribardière A, Centanni J, Dano A, Coudret J, Daguin-Thiébaut C, Houbin C, Kerdoncuff E, Jambut S, Cordaux R, and Broquet T (2018). Female-biased sex ratios unrelated to Wolbachia infection in European species of the Jaera albifrons complex (marine isopods). Journal of Experimental Marine Biology and Ecology 509: 91-98. [pdf]

  • Daguin-Thiébaut C and Broquet T (2018). Etude du polymorphisme par séquençage d'ADN associé à des sites de restriction (RAD-seq). In Principe des techniques de biologie moléculaire et génomique, Eds Tagu D, Jaubert-Paussamai S, and Méreau A.

  • Ribardière A, Daguin-Thiébaut C, Houbin C, Coudret J, Broudin C, Timsit O, and Broquet T (2017). Geographically distinct patterns of reproductive isolation and hybridisation in two sympatric species of the Jaera albifrons complex (marine isopods). Ecology & Evolution 7: 5352-5365 [pdf]

  • Riquet F, Comtet T, Broquet T, and Viard F (2017). Unexpected collective larval dispersal but little support for sweepstakes reproductive success in the highly dispersive brooding mollusc Crepidula fornicata. Molecular Ecology [pdf]

  • Eldon B, Riquet F, Yearsley J, Jollivet D, and Broquet T (2016). Current hypotheses to explain genetic chaos under the sea. Current Zoology 62: 551-566 [pdf].
  • Dussex N, Broquet T, and Yearsley J (2016). Contrasting dispersal inference methods using empirical data from the greater white-toothed shrew: implications for conservation. Journal of Wildlife Management and Wildlife Monographs 80:812-823 [pdf].
  • Yannic G, Broquet T, Strøm H, Aebischer A, Dufresnes C, Gavrilo M V, Mallory M L, Morrison R I G, Sabard B, Sermier R, Gilchrist H G, and Gilg O (2016). Genetic and morphological sex identification methods reveal a male-biased sex-ratio in the ivory gull Pagophila eburnea. Journal of Ornithology 157:861-873 [pdf].
  • Yannic G, Yearsley J, Sermier R, Dufresnes C, Gilg O, Aebischer A, Gavrilo M, Strøm H, Mallory M, Morrison G, Gilchrist G & Broquet T (2016). High connectivity in a long-lived High-Arctic seabird, the ivory gull Pagophila eburnea. Polar Biology 39: 221-236 [pdf]
  • Ribardière A, Broquet T, and Daguin-Thiébaut C (2015). Microsatellite markers for the Jaera albifrons species complex (marine isopods). BMC Research Notes 8:632 [pdf] [open pdf]
  • Broquet T, Barranger A, Billard E, Bestin A, Berger R, Honnaert G, and Viard F (2015). The size advantage model of sex allocation in the protandrous sex-changer Crepidula fornicata: role of the mating system, sperm storage, and male mobility. The American Naturalist 186:404-420 [pdf]
  • Gagnaire P-A, Broquet T, Aurelle D, Viard F, Souissi A, Bonhomme F, Arnaud-Haond S, and Bierne N (2015). Using neutral, selected, and hitchhiker loci to assess connectivity of marine populations in the genomic era. Evolutionary Applications 8:769-786 [pdf] [open pdf].
  • Luximon N, Petit E J, and Broquet T (2014). Performance of individual vs group sampling for inferring dispersal under isolation by distance. Molecular Ecology Resources 14: 745-752 [pdf] [open pdf]
  • Yearsley J, Viard F, and Broquet T (2013). The effect of collective dispersal on the genetic structure of a subdivided population. Evolution 67-6: 1649-1659 [pdf]
  • Broquet T, Viard F, and Yearsley J (2013). Genetic drift and collective dispersal can result in chaotic genetic patchiness. Evolution 67-6: 1660-1675 [pdf]
  • Yannic G, Basset P, Büchi L, Hausser J, and Broquet T (2012). Scale-specific sex-biased dispersal in the Valais shrew unveiled by genetic variation on the Y chromosome, autosomes, and mitochondrial DNA. Evolution 66-6: 1737-1750 [pdf]
  • Helfer V, Broquet T, and Fumagalli L (2012). Sex-specific estimates of dispersal show female philopatry and male dispersal in a promiscuous amphibian, the alpine salamander (Salamandra atra). Molecular Ecology 21: 4706-4720. [pdf]
  • Yannic G, Sermier R, Aebischer A, Gavrilo M V, Gilg O, Miljeteig C, Sabard B, Strøm H, Pouivé E, & Broquet T (2011). Description of microsatellite markers and genotyping performances using feathers and buccal swabs for the Ivory gull (Pagophila eburnea). Molecular Ecology Resources 11:877-889. [pdf]
  • Jaquiéry J, Broquet T, Hirzel A, Yearsley J and Perrin N. 2011. Inferring landscape effects on dispersal from genetic distances: how far can we go? Molecular Ecology 20: 692-705 [pdf]
  • Broquet T, Angelone S, Jaquiéry J, Joly P, Léna JP, Lengagne T, Plénet S, Luquet E and Perrin N. 2010. Genetic bottlenecks driven by population disconnection. Conservation Biology 24: 1596-1605. [pdf]
  • Jaquiéry J, Broquet T, Aguilar C and Perrin N (2010). Good genes drive female choice for mating partners in the lek-breeding European tree frog. Evolution 64: 108-115 [pdf]
  • Broquet T and Petit E (2009). Molecular estimation of dispersal for ecology and population genetics. Annual Review of Ecology, Evolution, and Systematics 40: 193-216 [pdf]
  • Broquet T, Jaquiéry J and Perrin N (2009). Opportunity for sexual selection and effective population size in the lek-breeding European tree frog (Hyla arborea). Evolution 63: 674-683 [pdf]
  • Broquet T, Yearsley J, Hirzel AH, Goudet J and Perrin N (2009). Inferring recent migration rates from individual genotypes. Molecular Ecology 18: 1048-1060 [pdf]
  • Jaquiéry J, Guélat J, Broquet T, Berset-Brändli L, Pellegrini E, Moresi R, Hirzel AH and Perrin N (2008). Habitat quality and metapopulation dynamics in the greater white-toothed shrew (Crocidura russula). Ecology 89: 2777-2785 [pdf]
  • Guélat J, Jaquiéry J, Berset-Brändli L, Pellegrini E, Moresi R, Broquet T, Hirzel AH and Perrin N (2008). Mass effects mediate coexistence in competing shrews. Ecology 89: 2033-2042 [pdf]
  • Berset-Brändli L, Jaquiéry, J, Broquet T and Perrin N (2008). Extreme heterochiasmy and nascent sex chromosomes in European tree frogs. Proceedings of the Royal Society of London B 275: 1577-1585 [pdf]
  • Berset-Braendli L, Jaquiéry J, Broquet T and Perrin N (2008). Isolation and characterization of microsatellite loci for the European tree frog (Hyla arborea). Molecular Ecology Resources 8: 1095-1097 [pdf]
  • Broquet T, Berset-Braendli L, Emaresi G and Fumagalli L (2007). Buccal swabs allow efficient and reliable microsatellite genotyping in amphibians. Conservation Genetics 8: 509-511 [pdf]
  • Broquet T, Ménard N and Petit E (2007). Noninvasive population genetics: a review of sample source, diet, fragment length and microsatellite motif effects on amplification success and genotyping error rates. Conservation Genetics 8: 249-260 [pdf]
  • Broquet T, Johnson CA, Petit E, Thompson ID, Burel F and Fryxell JM (2006) Dispersal and genetic structure in the American marten, Martes americana. Molecular Ecology 15: 1689-1697 [pdf]
  • Broquet T, Ray N, Petit E, Fryxell JM and Burel F (2006). Genetic isolation by distance and landscape connectivity in the American marten (Martes americana). Landscape Ecology 21: 877-889 [pdf]
  • Broquet T and Petit E (2004). Quantifying genotyping errors in non-invasive population genetics. Molecular Ecology 13: 3601-3608 [pdf]
  • Broquet T, Thibault M and Neveu A (2002). Distribution and habitat requirements of the white-clawed crayfish, Austropotamobius pallipes, in a stream from the Pays de Loire region, France. An experimental and descriptive study. Bull. Fr. Pêche. Piscic. 367: 717-728