Engineer

TECHNICAL MANAGER, KISSF SCREENING PLATFORM

        I began my career as a technician at the CNRS in animal experimentation in the field of infectious pathologies. In 2002, I was recruited to work as a technician and then as a research engineer on the functional genomics platform at the Institut Gustave Roussy. After this 10-year assignment, I joined the Station Biologique de Roscoff to work on a research project on protein phosphorylation and related pathologies. 

        My experience as an ISO9001-certified platform soon enabled me to devote myself to the development of the KISSf molecular screening platform. The process-based operating mode enabled me to define a strategy for continuous improvement.
        I invested in the refurbishment of all our equipment (dispensing robots, plate readers, benchtop -80, FPLC chain, thermophoresis) for a total budget of 500,000 euros. 
I developed the cold method process using bioluminescence to progressively eliminate radioactivity and enable better control of production costs.

        I have optimized the target production line to offer more targets for primary screening or selectivity. In 2 years, we have doubled our historical target library, developed over twenty years.     
Keen to pass on my experience, I'm involved in supervising trainees, particularly through apprenticeships. I'm currently supervising an apprentice in charge of developing thermophoresis methods. 

        Today, I'm the platform's technical manager. I supervise and coordinate the screening activities. We work with around twenty academic and private chemists.      
In parallel with this platform activity, I joined the TCCD team to work on the CDK10/CycM project coordinated by Dr Pierre Colas. Mutations in the kinase gene or its partner cyclin can lead to severe malformations and mental retardation. Our work aims to characterize this complex and gain a fundamental understanding of its mechanisms of action.

 

This year I'm organizing the "Roscoscreen 2.0" Thematic Screening School. I hope to see many of you at this event.  

 

Publications

  • 43 scientific publications, including two as 1st author on the CDK10/CycM project

Robert T, Dock-Bregeon AC, Colas P. Functional characterization of CDK10 and Cyclin M truncated variants causing severe developmental disorders Human Mutation : Manuscript submitted - humu-2021-0054

Robert T, Johnson JL, Guichaoua R, Yaron TM, Bach S, Cantley LC, Colas P. Development of a CDK10/CycM in vitro Kinase Screening Assay and Identification of First Small-Molecule Inhibitors. Front. Chem., 27 February 2020 | https://doi.org/10.3389/fchem.2020.00147.

Other publications are available for download:

PDF iconDownload thomas_robert_cv2_20240118.pdf (336.54 Ko)

 

SCIENTIFIC MEDIATION/COMMUNICATION

2021

Journée Break4tech/ Rencontre immersion “thème "Mer & Santé" à Roscoff”

Découverte et profilage d’inhibiteutrs de protéines kinasesà visée thérapeutique par Thomas ROBERT.

2020

Gentobio.

Mesure d’interaction entre ligands et cibles kinases par thermophorèse (MST, Microscale Thermophoresis) par Thomas ROBERT et Dorian LEFEBVRE, plate-forme KISSf.

2017

Gene2bio

Standardisation de la production et de la purification de protéines recombinantes : une étape importante en criblage in vitro par Thomas ROBERT et Mohamed SOUAB, Plate-forme KISSf.