Senior researcher CNRS

Identité Laurence Garczarek

I am a research scientist at CNRS since 2003 and leader of the MaPP team. My main research interest lies in understanding the genetic and functional diversity of marine picocyanobacteria and the consequences on their adaptation/acclimation capacity to various environmental factors

 

MAJOR CURRENT RESEARCH PROJECTS

  • ANR CINNAMON, Analyse multi-échelles de l’adaptation à la carence en Fer chez un organisme clé du phytoplancton marin, dans un contexte de changement global (2018-2020, PI: L Garczarek)
  • ANR SAMOSA, Synechococcus as a model genus for studying adaptation of marine phytoplankton to environmental changes (2014-2018, PI: L Garczarek)
  • MetaSyn Genoscope, Metagenomic study of Synechococcus phycobilisomes (PI: L Garczarek)
  • Program IE EMBRc: European Marine Biological Resource Centre
  • Flagship Project INPUT: Integrative Pelagic Ecosystem Responses to nUtrients fertilization and microplasTics addition (2016-2018, PI: C. Brunet)

 

MAIN SCIENTIFIC INTEREST

  • Diversity and ecology of marine photosynthetic prokaryotes
  • Genomics, genetics and evolution of marine picocyanobacteria
  • Physiology and regulation of the response to environmental stresses

 

COLLABORATIONS

  • Damien Eveillard, University of Nantes, France
  • Anne Siegel, IRISA Rennes, France
  • Silvia Acinas, ICM−CSIC, Spain
  • David J. Scanlan, Univeristy of Warwick, UK
  • David Kehoe, Indiana University, USA

 

PAST PHD STUDENTS

  • 2011-2015 Gregory Farrant, UPMC
  • 2009-2013 Florian Humily, UPMC
  • 2007-2010 Daniella Mella, UPMC
  • 2006-2010 Christian Kolowrat (co-direction with F. Partensky)
  • 2004-2006 Ludovic Joubin, UPMC

 

PAST POST-DOCS

  • 2014-2016 Ngoc AN Nguyen
  • 2006-2008 Christophe Boutte
  • 2006-2008 Nicolas Blot (co-direction with F. Partensky)
  • 2003-2006 Julia Holtzendorff (co-direction with F. Partensky)

 

 

Publications

GENETIC DIVERSITY AND GEOGRAPHICAL DISTRIBUTION

• Xia X., Partensky F., Garczarek L., Suzuki K., Guo C., Cheung S.Y. & Liu H.: 2017.
Phylogeography and pigment type diversity of Synechococcus cyanobacteria in surface waters of the northwestern Pacific Ocean. Environ. Microbiol.19: 142–158.  http://doi.org/10.1111/1462-2920.13541

• Paulsen M.L., Bratbak G., Doré H., Garczarek L., Seuthe L., Müller O., Sandaa R.H., Larsen A. (2016).
Synechococcus in the Atlantic gateway to the Arctic Ocean. Frontiers in Marine Science  http://doi.org/10.3389/fmars.2016.00191.

• Farrant GK, Doré H, Cornejo-Castillo FM, Partensky F, Ratin M, Ostrowski M, Pitt FD, Wincker P, Scanlan DJ, Iudicone D, Acinas SG, Garczarek L (2016)
Delineating ecologically significant taxonomic units from global patterns of marine picocyanobacteria. Proc Natl Acad Sci USA doi/10.1073/pnas.1524865113

• Villar E, Farrant G, Follows M, Garczarek L, Speich S, Audic S, Bittner L, Blanke B, Brum JR, Brunet C, et al. 2015.
Environmental disturbance in Agulhas rings affects inter-ocean plankton dispersal. Science 348: 6237 p1261447-1.

• Mella-Flores D., Mazard S., Humily S., Partensky F., Mahé F., Bariat L., Courties C., Marie D., Ras J., Mauriac R., Jeanthon C., Bendif E.M., Ostrowski M., Scanlan D.J., Garczarek L. 2011.
Is the distribution of Prochlorococcus and Synechococcus ecotypes in the Mediterranean Sea affected by global warming? Biogeosciences 8, 2785–2804.

• Partensky, F. and Garczarek L. 2011.
Arms race in a drop of sea water. Nature 474:582-583. (commentaire invité d'article dans la section "News and Views")

• Garczarek, L., Hess, W., Holtzendorff, J., van der Staay, G.W.M., Partensky, F., 2000.
Multiplication of antenna genes as a major adaptation to low light in a marine prokaryote. Proceedings of the National Academy of Sciences of USA 97: 4098 -4101

 

DEVELOPMENT OF METAGENOMIC APPROACH

• Mazard, S., Ostrowski, M., Garczarek,, L., Scanlan, D.J. 2011.
A targeted metagenomic approach to determine the ‘population genome’ of marine Synechococcus. In Handbook of Molecular Microbial Ecology II: Metagenomics in Different Habitats. F. J. de Bruijn (Editor).

• Humily F. Farrant, G.K., Marie D., Partensky F., Mazard S., Perennou M., Labadie K., Aury J.M., Wincker P., Nicolas Segui A., Scanlan D.J. and Garczarek L. 2014.
Development of a targeted metagenomic approach to study a genomic region involved in light harvesting in marine Synechococcus. FEMS Microbiol. Ecol., 88 231–249.

 

PHYSIOLOGY

• Pittera J., Humily F, Ratin M., Thorel M. ., Garczarek L., & Six C. 2014.
Connecting thermal physiology and latitudinal niche partitioning in marine Synechococcus. ISME J. 1–16.

• Mella-Flores D., Six C., Ratin M., Partensky  F., Boutte C., Le Corguillé G., Marie D., Blot N., Gourvil P., Kolowrat C., Garczarek L. 2012.
Prochlorococcus and Synechococcus have evolved different adaptive mechanisms to cope with light and UV stress. Frontiers in Aquatic Microbiology 3:285.

• Blot, N., D. Mella-Flores, C. Six, G. Lecorguille, C. Boutte, A. Peyrat, A. Monnier, M. Ratin, P. Gourvil, D. A. Campbell and L. Garczarek. 2011.
Light history influences the response of the marine cyanobacterium Synechococcus sp. WH7803 to oxidative stress. Plant Physiology 156: 1934-1954 (FI= 6.451).

• Blot, N., Wu, X.-J., Thomas, J.-C., Zhang, J., Garczarek, L., Böhm, S., Tu, J. M., Zhou, M., Plöscher, M., Eichacker, L., Partensky, F., Scheer, H. & Zhao, K. H. 2009.
Phycourobilin in a unique trichromatic phycobiliprotein is formed post-translationally by a novel phycoerythrobilin lyase-isomerase. Journal of Biological Chemistry 284:9290–9298.

• Garczarek, L., Dufresne, A., Blot, N., Cockshutt, A.M., Peyrat, A., Campbell, D.A., Joubin, L & Six, C. 2008.
Function and evolution of the psbA gene family in marine Synechococcus: Synechococcus sp. WH7803 as a case study. ISME journal.

 

COMPARATIVE GENOMICS

• Farrant, GK, Hoebeke, M., Partensky, F., Andres, G. and Garczarek, L. 2015.
WiseScaffolder: an algorithm for the semi-automatic scaffolding of Next Generation Sequencing data. BMC Bioinformatics 16:281.

• Dufresne, A., Ostrowski, M., Scanlan, D. J., Garczarek, L., Mazard, S., Palenik, B. P., Paulsen, I. T., Tandeau de Marsac, N., Wincker, P., Dossat, C., Ferriera, S., Johnson, J., Post, A. F., Hess, W. R. & Partensky, F. 2008.
Unraveling the genomic mosaic of a ubiquitous genus of marine cyanobacteria. Genome Biology 9:R90. 16pp (FI=6,153).

• Six C., Thomas J.-C., Dufresne A., Garczarek L., Ostrowski M., Scanlan D.J., Arrouy N. & Partensky F. 2007.
Genes encoding phycobilisome components of marine Synechococcus cyanobacteria have evolved independently from the core genome. Genome Biology 8(12):R259, 22pp (FI=6,153).

• Dufresne, A., Garczarek L. et Partensky F. 2005.
Accelerated evolution associated with genome reduction in a free-living prokaryote. Genome Biology 6:R14 (10 pp.) (IF: 6.153).

 

PIGMENT TYPES DIVERSITY AND DISTRIBUTION

• Mahmoud RM., Sanfilippo J.E, Nguyen A.A., Strnat J.A., Partensky F., Garczarek L., El-Kassem N.A. Kehoea D.M. and Schluchter M. (2017).
Adaptation to blue light in marine Synechococcus requires MpeU, an enzyme with similarity to phycoerythrobilin lyase isomerases. Frontiers in Microbiology (section Microbial Physiology and Metabolism).  http://doi.org/10.3389/fmicb.2017.00243

• Sanfilippo JE, Nguyen AA, Karty JA, Shukla A, Schluchter WM, Garczarek L, Partensky F, Kehoe DM (2016).
A self-regulating genomic island encoding tandem regulators confers chromatic acclimation to marine Synechococcus. Proc Natl Acad Sci USA 113(21):6077-82.

• Humily F. Partensky F., Six C., Farrant, G.K., Ratin M., Marie D., and Garczarek L. 2013.
A gene island with two possible configurations is involved in chromatic acclimation in marine Synechococcus. PLOS One, vol 8, issue 12, e84459.

• Kolowrat, C., Partensky, F., Mella-Flores, D., Le Corguillé, G., Boutte, C., Blot, N., Ratin, M., Ferréol, M., Lecomte, X., Gourvil, P., Lennon, J.F., Kehoe, D. and Garczarek, L.
Ultraviolet stress delays chromosome replication in light/dark synchronized cells of the marine cyanobacterium Prochlorococcus marinus PCC9511. BMC Microbiology 10:204

 

REVIEW

• Hess W.R., Garczarek L., Pfreundt U. & Partensky F. 2016.
Phototrophic Microorganisms: The Basis of the Marine Food Web. In: The Marine Microbiome: An Untapped Source of Biodiversity and Biotechnological Potential (Stal LJ, Cretoiu MS eds), pp. 57-97. Springer International Publishing: Cham,   http://doi.org/10.1007/978-3-319-33000-6_3

• Partensky, F. & Garczarek, L. 2010.
Prochlorococcus: Advantages and limits of minimalism. Annual review of Marine Science 2:305–31.

• Scanlan, D. J., Ostrowski, M., Mazard, S., Dufresne, A., Garczarek, L., Hess, W. R., Post, A. F., Hagemann, M., Paulsen, I. & Partensky, F. 2009.
Ecological genomics of marine picocyanobacteria. Microbiology and Molecular Biology Reviews 73: 249-299 (FI= 16,950).