CristalO - crystallography core facility | Core Facilities

Crystallogenesis and Structure Determination

The crystallogenesis service involves producing crystals from a soluble protein. Crystallization conditions are automatically identified using a crystallization robot before the manual steps of reproducing and optimizing the crystals.

Structure determination is then performed using protein crystallography, which consists of exposing the crystals to synchrotron X-rays to obtain diffraction, collecting the data, and carrying out the resolution and refinement of the 3D coordinates.

Infrastructure de recherche

Réseaux

Contact(s)

Alexandra Jeudy
jeudy@sb-roscoff.fr

Services

We make our expertise available to provide you with the following services:

Determination of the oligomeric state of a sample by DLS
Sample crystallization using a Crystal Gryphon crystallization robot
Monitoring of crystallization with a CrysCam camera and/or a stereomicroscope
Manual reproduction and optimization of crystallization conditions
Determination of cryo-freezing conditions for crystals
X-ray diffraction of crystals
Data collection and processing
Phasing, reconstruction, and refinement of the 3D structure

Our expertise focuses on biomolecules, namely:

• Soluble proteins
• Protein complexes including hydrophilic and hydrophobic molecules
• Protein–protein complexes
• Protein–nucleic acid complexes

DLS Litesizer 700 - Anton Paar

Estimer la taille et la concentration de particules comprises entre 0,3 nm et 10 μm dans un mélange. Déterminer le potentiel Zeta d'une protéine. Automatisation des mesures avec variabilité du pH, concentration en tampon et sel.

Robot nano-gouttes : Art Robbins Crystal Gryphon

Le robot nano-goutte Gryphon automatise la préparation de gouttes de cristallisation à très faible volume dans des plaques 96 puits. Il utilise la technique de diffusion en phase vapeur en goutte assise, garantissant précision, rapidité et reproductibilité dans le criblage des conditions de cristallisation.