Research engineer

Specialized in flow cytometry

Dominique Marie

current position

In charge of the flow cytometry at the Station Biologique of Roscoff that is equipped with a high speed cell sorter (FACS Aria, Becton Dickinson) installed in 2003, and 4 analyzers:  (FACS Canto II, Becton Dickinson, 2008), Cell Lab Quanta (Beckman coulter, 2009), Accuri C6 (Becton Dickinson 2011) and Guava EasyCyte HT (Millipore 2016).

This responsibility involves the maintenance of flow cytometers, users management and training.

My tasks include the participation to oceanographic cruises, the analysis of natural samples or cultures, the data analysis, the presentation and publication of the results. I'm also in charge of the methodological developments related to flow cytometry in the marine domain and physical sorting of cells by flow cytometry for molecular biology purposes or to obtain microalgae in culture.

 

background

I joined the CNRS at the end of 1981 and worked for eleven years in very different fields: microbiology, organic chemistry, medical and plant biology. This allowed me to be confronted with a wide range of techniques including flow cytometry, technique already widespread in the biomedical field at this time, but not really developped in the field of marine ecology.

 

In 1992, Daniel Vaulot (CNRS DR) convinced me to join him at the Station Biologique of Roscoff, in the team Plancton Océanique that was composed of 4 people including 2 permanents (it is curently composed of 35 people including 10 researchers. He gave me the responsibility of the cytometry platform that he wanted to develop for the study of marine plankton.

 

In the frame of my mission I had to participate to oceanographic cruises when a flow cytomètre was embarked. I participated to oceanographic missions or workshops  like OLIPAC: 1994; MINOS: 1996 et PROSOPE: 1999; PROMOLEC: 2000; BIOSOPE 2004; MALINA 2009, CEFAS 2011, Green Edge 2016).

 

Part of my work consists in training technicians and researchers abroad (Israel, Chile, Norway, Iceland) in the field of flow cytometry applied to marine samples.

 

I also taught cytometry in microbiology and marine biology (Montpellier, Clermont-Ferrand, Roscoff).

 

Publications

Until this day (May 2019), I have been implied in 131 publications, among which 111 are ranked A, as main or co-author.

Guillot L., Delage L., Viari A., Vandenbrouck Y., Com E., Ritter A., Lavigne R., Marie D.,  Peterlongo P., Potin P. and Pineau C. 2019. Peptimapper: proteogenomics workflow for the expert annotation of eukaryotic genomes. BMC Genomics  20:56. https://doi.org/10.1186/s12864-019-5431-9

Demory, D., Baudoux, A., Monier, A., Simon, N., Six, C., Ge, P., Marie, D. et al. 2019. Picoeukaryotes of the Micromonas genus : sentinels of a warming ocean. ISME J. Vol.13, Issue 1, pages 132-146

S. A. Bulat, M. V. Doronin, G. P. Pavlov, D. S. Karlov, D. Marie and J.-R. Petit. 2018. Unknown Widespread Iron- and Sulfur-Oxidizing Bacteria beneath the East Antarctic Ice Sheet. ISSN 0031-0301, Paleontological Journal, Vol. 52, No. 10, pp. 1196–1203

Partensky F., Mella-Flores D., Six C., Garczarek L., Czjzek M., Marie D., Kotabová E., Felcmanová K. and Prášil O. 2018. Comparison of Photosynthetic Performances of Marine Picocyanobacteria with Different Configurations of the Oxygen Evolving Cluster. Photosynthesis Research 138: 57-71. https://doi.org/10.1007/s11120-018-0539-3

Gérikas Ribeiro C., Lopes dos Santos A., Marie D., Pereira Brandini F. and Vaulot D. 2018. Small eukaryotic phytoplankton communities in tropical waters off Brazil are dominated by symbioses between Haptophyta and nitrogen-fixing cyanobacteria. The ISME Journal https://doi.org/10.1038/s41396-018-0050-z

Partensky F., Six C., Ratin M., Garczarek L., Vaulot D., Probert I., Calteau A., Gourvil P., Marie D., Grébert T., Bouchier C., Le Panse S., Gachenot M., Rodríguez F. and Garrido J.L. 2018. A novel species of the marine cyanobacterium Acaryochloris with a unique pigment content and lifestyle. Nature Scientific Reports (2018) 8:9142 | DOI:10.1038/s41598-018-27542-7