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Since 28 jan 2005
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Home page arrow Research arrow Resources arrow CytoWin - a flow cytometry analysis software

CytoWin - a flow cytometry analysis software Print
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Written by Daniel Vaulot   
Friday, 28 January 2005
CYTOWIN is a Windows 3.1 program designed to analyse flow cytometer data. CYTOWIN can analyse flow cytometer data in one of the following forms : single parameter histograms, two-parameter cytograms and list mode. It can analyze data generated by the following instruments:
  • Coulter: EPICS 5, Profile or Elite
  • Becton Dickinson: FACScan, FACSort, FACSAria
  • Cytomation CICERO.

It can also analyse other distributions stored as ASCII files such as Coulter Counter volume distributions. Its main advantages are the speed of processing and the possibility to directly recover statistics in worksheet format.

 

CYTOWIN has been written by D. Vaulot from CNRS, Station Biologique, BP 74 29682 Roscoff Cx FRANCE, with help from Jeff Dusenberry (MIT). It is free of charge provided it is used for scientific purposes and provided the following citation is used in scientific papers:

  • Vaulot, D. 1989. CYTOPC: Processing software for flow cytometric data. Signal and Noise 2:8

 

You can also obtain information on analysis protocols in the following chapter:

  • Marie D., Brussaard C., Partensky F. and Vaulot D. Flow cytometric analysis of phytoplankton, bacteria and viruses. 1999. In: Current Protocols in Cytometry. John Wiley & Sons, Inc. 11.11.1-11.11.15.

Latest versions (available in the download section )

  • Cytowin 4.31 - Compiled November 2004
    • CytoWin can read FACSAria files saved as FCS2.0 in 1024 channels (cannot read native FACSAria files or FCS 3.0). [Note if you have Version 4.2 please upgrade to 4.31 since BD Aria software has changed the way files are exported to FCS format)
    • Maximum number of parameters extended to 12 including Time.
    • Configuration file format has changed since the previous version (4.1). Therefore they cannot be directly read. Instead use the Menu Item “Import Configuration File 4.1 or older”.

 

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Last Updated ( Friday, 20 October 2006 )
 
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