PICODIVa European FP5 program
Monitoring the diversity of photosynthetic picoplankton in marine waters

Meeting 2001

Results from Year 1
Planning Year 2

2 May - 4 May 2001, ICM, Barcelona, Spain

Meeting organizer. Daniel Vaulot, SBR

The format of the 2001 meeting followed closely that of 2000.  During the first two days results obtained in the different workpackages have been presenteed under the format of scientific talks.  Then in the last day, we discussed planification for year 2. You can download Power presentations by clicking on the title of the talk (be careful, some of these files are very big...).
  • Minutes of meeting

Results from year 1
Wednesday 2May
The aim of this first part is to present an exhaustive view of the work performed during the first year of PICODIV. Please feel free to add anything that could be of interest for the project.
   9:00 Vaulot Introductory remarks
Picoplankton diversity from cultures
   9:30 Le Gall Current status of the RCC collection
10:00 Romari Screening of cultures by RFLP and sequences of rDNA - 15 min.
10:30 Eikrem and Throndsen EM of novel species isolated in culture (big file 23 Mb...) - 20 min.
11:00 Coffee Break
11:15 Latasa HPLC analysis of novel cultures - 20 min
  11:45 Guillou Diversity obtained in culture from the Blanes site screened by DGGE - 30 min
12:15 Lunch Break
   13:30 Scanlan Marine cyanobacteria cultures: Prochlorococcus and Synechococcus
   14:00 Vaulot et al Synthetic overview of potentially interesting/novel cultures in the RCC collection - 20 min
Picoplankton diversity from clone libraries and other molecular approaches
14:30 Guillou Diversity of the eukaryotes and prokaryotes (including plastids) assemblages assessed from fine size fractionation and DGGE. - 20 min.
  15:00 Scanlan Prokaryotic and plastid picoplankton clone libraries - 15 min
  15:30 Coffee break
15:45 Valentin Helgoland and Atlantic clone libraries
  16:10 Romari Roscoff clone libraries
16:45 Massana Clone libraries from the Blanes site, Mediterranean Sea - 20 min.
17:15 Díez A spatio-temporal comparison of picoeukaryotes in the Alborán Sea (SW Mediterranean) by denaturing gradient gel electrophoresis and HPLC pigment analysis
  17:45 Díez Description and variability of Antarctic picoeukaryotic assemblages studied by two fingerprintins methods (DGGE and T-RFLP) - 20 min.
Thursday 3 May
Databases
9:00 Vaulot Overview of PICODIV web site and databases
Probe measurement and development
Methods 9:30 Not Optimisation of the FISH-TSA protocol for picoplankton
   9:45 Biegala, Marie Coupling FISH-TSA with flow cytometry
  10:15 Biegala Quantitative PCR: plans
10:30 Coffee break
  11:00 Valentin, Medlin High density arrays and DNA chips: plans - 15 min
 Probes 11:30 Medlin Overview of probe development - 20 min
  12:00 Not Probes for Prasinophytes
12:15 Massana Probes for Stramenopiles: design and testing - 15 min.
  12:30 Fuller Probes for Synechococcus (Part 1, Part 2)- 15 min
13:00 Lunch break
Coastal sampling sites
  14:30 Not and Simon The Roscoff site
  15:00 Valentin The Helgoland site with discussion of culture isolation
  15:30 Massana The Blanes Bay site - 15 min
16:00 Coffee break
Analysis of natural populations
16:30 Fuller Prochlorococcus dynamics in the Red Sea - an annual cycle - 30 min
(Part 1, Part 2)
  17:00 Eikrem  Electron microscopy analysis of natural populations


Planning PICODIV year 2
Friday 4 May
   9:00 Workpackage 1
Daniel, Wenche		 Cultures
  • Isolation needs to be continued especially for the Helgoland site from which we have very little cultures.
  • Isolation methods (flow cytometry, serial dilution, plating)
  • Screening methods (HPLC, rDNA, microscopy)
  • Which cultures should be selected for full study (criteria to set priorities: novelty, picoplanktonic size) and formal description
   10:00 Workpackage 2
Dave, Klaus, Ramon		 Clones libraries
  • Solve problem of cyanobacteria clone libraries
  • Screening of samples prior to clone libraries by DGGE
  • Should we do more eukaryotic clone libraries? Where ?
    • Bloom evolution (Roscoff)
    • Oslo fjord in conjunction with TEM
    • PROSOPE cruise after screening of samples
    • Red Sea from 2000 workshop
    • Antarctic
  • Should we probe clone libraries from underrepresented groups (cf Haptophytes etc...)
  • Which clones should we completely sequence?
   11:00 Workpackages 3-4
Linda, Dave		 Probes
  • List of groups to be targeted
    Suggestion: for FISH it would be best to only have Class level probes while for DNA chips we could have both class level and species specific probes knowing that we have no limitation in terms of the number of probes that can be used. 
  • List of probes to be designed
    • Classes
    • Genus, species
  • What will be the focus of each partner in terms of probe validation
  • Probe detection:
    • FISH by microscopy and flow cytometry
    • Quantitative PCR
    • Dot blot
    • DNA chips
   14:00 Workpackage 5
Carlos		 Sampling
  • Optimisation of sample collection in particular for TEM
  • Optimisation of sampling operation (cf Helgoland)
  • Which minimum set of probes should we use on the collected samples
  • Colloboration with other groups (eg Helgoland: DMS and PUFA)
15:30 First annual report due May 30
   16:00 Planned publications and Meeting presentations
    Arrangements for next meeting

Last updated 16 May 2001

Send comments/corrections to Daniel Vaulot