PICODIVa European FP5 program
	Monitoring the diversity of photosynthetic picoplankton in marine waters

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Startup meeting

28-29 June 2000, Station Biologique, Roscoff, France

By clicking on speaker name you will download presentation (PowerPoint or Word).

Wednesday 28 June
Where are we at the start of PICODIV?
The aim of this first part is to present an exhaustive view of the state of the art and of any PICODIV related work that has begun prior to the meeting. Please feel free to add anything that could be of interest for the project.
	9:00	Vaulot	Introductory remarks
What is known about picoplankton diversity
	9:10	Throndsen	An introduction to picoplankton taxonomy
	10:00	Scanlan	Diversity of marine cyanobacteria: Prochlorococcus and Synechococcus
	10:30	Guillou	Diversity of picoplanktonic Prasinophytes
	11:00	Coffee break
Coastal sampling sites
	11:30	Simon et al.	Presentation of the Roscoff site, summary of the historical data since 1985
	12:00	Scharek	Presentation of the Helgoland site
	12:30	Massana	Presentation of the Blanès Bay site Presentation of HIVERN cruises Presentation of ARO cruise
	13:00	Lunch
Other sites and cruises
	14:00	Partensky et al.	Overview of the PROSOPE cruise (Mediterranean Sea), September 1999 with emphasis on picoplankton
	14:30	Scanlan	The Red Sea sampling site: cyanobacteria dynamics.
Databases
	14:45	Vaulot	Database implementation Taxonomy Cultures Environmental data Probes Sequences
Cultures
	15:30	Le Gall et al.	Culturing strategy: initial results from the screening of PROSOPE and Roscoff cultures
	16:00	Tea break
Ancilary techniques: flow cytometry, HPLC, TEM
	16:30	Marie et al.	Update on flow cytometry approaches for picoplankton
	17:00	Latasa	Latest development in HPLC and first overview of fractionated samples from Roscoff
	17:30	Eikrem et al.	TEM methodology and initial results on grids from PROSOPE
Thursday 29 June
Molecular approaches
	9:00	Valentin et al.	Optimizing DNA extraction and cloning efficiency for picoplankton samples First results from Helgoland clone library
	9:30	Scanlan	Strategies to study Prochlorococcus molecular ecology
	9:45	Diez et al.	DGGE analysis of eukaryotic picoplankton (see ppt file on primers)
	10:00	Medlin	Overview of probes available for eukaryotic picoplankton. Probe design and probe measurement: high density arrays and DNA chips
	10:30	Not	Coupling FISH-TSA with eukaryotic probes for picoplankton: initial results for the Roscoff site.
	11:00	Coffee break
			Free
	13:00	Lunch
Planning PICODIV
Click here for Minutes of Meeting
	14:00	Workpackage 1 Daniel, Wenche	Cultures Isolation methods Central repositary sites
	15:00	Workpackage 2 Dave, Klaus, Ramon	Methods Cloning vector Primers choice for short runs and full sequences PCR enzymes, PCR cycles, thermocyclers Should we negotiate with a single company to subcontract sequencing Companies for other items (oligos, enzymes) Programs used for sequence analysis, aligning etc: local Blast, ARB Programs used for phylogenetic analyses including parameter settings (Weighting of gaps, positions, distance matrices etc.) Clones libraries Coastal Site Equatorial Pacific (OLIPAC) Mediterranean Sea (MINOS, PROSOPE, HIVERN, MATER) North Atlantic (Orkney Islands, Celtic Sea) Red Sea Southern Ocean ?
	16:00	Tea Break
	16:30	Workpackages 3 Linda, Dave, Nathalie	Probes List of probes to be designed Classes Clades Genus, species Probe detection: FISH Dot blot DNA chips
	17:30	Workpackages 4 Carlos, Nathalie, Mikel	Sampling Filtration, volumes etc... DNA/RNA extraction protocols TEM protocols
	18:30		Arrangements for next meeting
	18:50	Adjourn

Last updated 20 January 2001