PICODIVa European FP5 program
	Monitoring the diversity of photosynthetic picoplankton in marine waters

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Workshop

Microbial Diversity in Aquatic Systems:
Environmental Gene Cloning and Analysis

25- 27 June 2000, Station Biologique, Roscoff, France

• List of papers on the various techniques discussed during the workshop (DGGE, SSCP etc...)
• Useful web sites
• Pictures taken during the workshop ( Banquet pictures)
• Book of abstracts (Download in pdf format)
• List of participants
• Preliminary schedule
• Issues to be discussed

List of participants

Alazard	Didier	IRD Marseille	Didier.alazard@esil.univ-mrs.fr
Barbrook	Adrian	Dept of Biochemistry, Cambridge University	acb18@mole.bio.cam.ac.uk
Benlloch	Susana	Universidad Miguel Hernández. División Microbiología.	susana.benlloch@umh.es
Biegala	Isabelle	Station Biologique Roscoff	biegala@sb-roscoff.fr
Blanchot	Jean	Station Biologique Roscoff	blanchot@sb-roscoff.fr
Castberg	Tonje	Dep. of Microbiology, Univ of Bergen	tonje.castberg@im.uib.no
Cauchie	Henry	CRP-GL CREBS	cauchie@crpgl.lu
Charpy	Loïc	IRD, COM Marseille	lcharpy@com.univ-mrs.fr
Courties	Claude	Laboratoire Arago Banyuls	courties@obs-banyuls.fr
Daae	Frida Lise	Dep. of Microbiology, Univ of Bergen	frida.daae@im.uib.no
Díez	Beatriz	ICM Barcelona	bdiez@icm.csic.es
Edvardsen	Bente	University of Oslo, Department of Biology, Section for Marine Botany	bente.edvardsen@bio.uio.no
Eikrem	Wenche	University of Oslo, Department of Biology, Section for Marine Botany	wenche.eikrem@bio.uio.no
Engelen	Bert	GBF - Division of Microbiology Braunschweig	ben@gbf.de
Fardeau	Marie-Laure	IRD Marseille	fardeau@esil.univ-mrs.fr
Felman	Héloïse	Station Biologique Roscoff	felman@sb-roscoff.fr
Ferris	Michael	Montana State University	mferris@montana.edu
Guillou	Laure	IFREMER Brest	Laure.Guillou@ifremer.fr
Howarth	Richard	University of Warwick
Kooistra	Wiebe	Marine Biology, RuG	W.H.C.F.Kooistra@biol.rug.nl
Latasa	Mikel	ICM Barcelona	latasa@icm.csic.es
Le Gall	Florence	Station Biologique Roscoff	flegall@sb-roscoff.fr
Lebaron	Philippe	Laboratoire Arago Banyuls	lebaron@arago.obs-banyuls.fr
Marie	Dominique	Station Biologique Roscoff	marie@sb-roscoff.fr
Marsh	Terence	Ctr. Microbial Ecology, Michigan State University	MARSHT@pilot.msu.edu
Massana	Ramon	ICM Barcelona	ramonm@icm.csic.es
Medlin	Linda	Alfred-Wegener-Institute, Bremerhaven	lmedlin@awi-bremerhaven.de
Mehl	Helga	Alfred-Wegener-Institute, Bremerhaven	hmehl@awi-bremerhaven.de
Mugnai	Maria Angela	Centro Studio Microrganismi Autotrofi-CNR Florence	m.mugnai@csma.fi.cnr.it
Munguia Vega	Adrian	CIBNOR, Marine Pathology, La Paz	airdrian@cibnor.mx
Muyzer	Gerard	Netherlands Institute for Sea Research	gmuyzer@nioz.nl
Nercessian	Olivier	UMR6539-CNRS-LEMAR Brest	olivier.nercessian@univ-brest.fr
Not	Fabrice	Station Biologique Roscoff	not@sb-roscoff.fr
Orsini	Luisa	Stazione Zoologica, Napoli	orsini@alpha.szn.it
Palenik	Brian	SIO/Univ. of Calif. San Diego	bpalenik@ucsd.edu
Partensky	Frederic	Station Biologique Roscoff	partensk@sb-roscoff.fr
Pedrós-Alió	Carlos	ICM Barcelona	cpedros@icm.csic.es
Rajaniemi	Pirjo	Helsinki University, Department of chemistry and microbiology	pirjo.rajaniemi@helsinki.fi
Rantala	Anne	University of Helsinki, Department of Applied Chemistry and Microbiology	Anne.Rantala@helsinki.fi
Romari	Khadidja	Station Biologique Roscoff	romari@arpb.univ-montp2.fr
Scanlan	Dave	University of Warwick	dp@dna.bio.warwick.ac.uk
Scharek	Renate	Alfred-Wegener-Institute, Helgoland	rscharek@awi-bremerhaven.de
Simon	Nathalie	Station Biologique Roscoff	simon@sb-roscoff.fr
Sjoling	Sara	University College London	s.sjoling@ucl.ac.uk
Tebbe	Christoph C.	Institute for Agroecology Braunschweig	christoph.tebbe@fal.de
Throndsen	Jahn	University of Oslo, Department of Biology, Section for Marine Botany	jahn.throndsen@bio.uio.no
Tichy	Martin	University of South Bohemia	tichym@tb.bohem-net.cz
Valentin	Klaus	Alfred-Wegener-Institute, Bremerhaven	kvalentin@awi-bremerhaven.de
Vaulot	Daniel	Station Biologique Roscoff	vaulot@sb-roscoff.fr
Vazquez-Juarez	Ricardo	CIBNOR, Marine Pathology, La Paz	rvazquez@cibnor.mx
West	Nyree	University of Warwick	mmqw@dna.bio.warwick.ac.uk
Wilmotte	Annick	Université de Liège	awilmotte@ulg.ac.be
Zandvliet	Dorothea	St George's Hospital Medical School	d.zandvliet@sghms.ac.uk

Schedule

(see final schedule in book of abstracts)

Sunday 25 June

09.00-09.10 D. Scanlan Opening Remarks

Session 1 Techniques for assessing diversity and community structure of microbial populations

09.10-10.00 G. Muyzer Use of DGGE in analysing microbial community structure

10.00-10.50 M. Ferris Insights into microbial community structure revealed by molecular analyses of population distributions along environmental gradients using cloning and DGGE

Coffee Break 1050-1120

11.20-12.10 C. Tebbe Use of PCR-SSCP (single strand conformation polymorphism) in Microbial Ecology

12.10-12.35 B. Engelen Fingerprinting bacterioplankton of the central Baltic by 16S rDNA community analysis

12.35-13.00 P. Lebaron P. and C. Courties Cell sorting and molecular biology for diversity assessment

Lunch 13.00-14.30

14.30-15.20 T.L. Marsh, F. Crocker, M.S. Riley, and B. Wade Practical Aspects and Pitfalls of Microbial Community Analysis with T-RFLP

15.20-15.45 S. Sjoling and D. Cowan Diversity of prokaryotes and direct cloning of novel enzyme genes from Antarctic marine environment.

15.45-16.15 Tea Break

16.15-16.45 C. Tebbe Dry demonstration of the SSCP technique

16.45 Demonstration/ use of DGGE & HA Yellow/Red gels

Evening: Informal discussions

Monday 26 June

0900- 0935 Visualisation of DGGE gel DGGE results/comments/discussion

Session 2 Diversity of Prokaryotic Picoplankton in Aquatic Environments:

09.35-10.25?B. Palenik: Diversity of Marine Cyanobacteria

10.25-10.50 ?F. Partensky , L. Garczarek and W.R. Hess Molecular bases of ecotypic diversity in Prochlorococcus

10. 50-11.20 Coffee Break

11.20-11.45 L. Charpy Prokaryotic picophytoplankton in tropical coastal lagoons

11.45-12.10 P. Rajaniemi, A. Rantala, K. Haukka, K. Sivonen Diversity of cyanobacteria in the Finnish lake environment

12.10-12.35 S. Benlloch, J. Garcia-Martinez, A. Lopez-Lopez, P. Lopez-Garcia and F. Rodriguez-Valera Prokaryotic biodiversity in aquatic marine environments.

12.35-1300 O. Narcessian Assessment of microbial diversity in a deep hot oil reservoir from Western Siberia by molecular methods

Lunch 13.00- 14.30

Session 3 Molecular markers for identifying and exploring biodiversity amongst eukaryotic picophytoplankton

14.30-14.55 L. Guillou, M. A. Cambon, & G. Barbier. Genetic diversity of the French Dinophysis, Alexandrium and Gymnodinium genera based upon direct cells amplification on fixed cultures or natural samples.

14.55- 15.20 B. Edvardsen, K. Shalchian-Tabrizi, S. Brubak, E. Dahl, K.S. Jakobsen, L.K. Medlin & E. Paasche Genetic analyses of Dinophysis species isolated from Norwegian waters

15.20-15.45 T. Castberg Multi-method approach to study marine microbial ecology. Examples from a mesocosm experiment with an Emiliania huxleyii bloom terminated by virus.

15.45-16.15 Tea break

1615- Demonstration of FISH (introduction to confocal microscope etc)

1630- 1700 N West Use of tyramide signal amplification in fluorescent in situ hybridisation technology to detect marine cyanobacteria

1730 -1900

• N. Simon/F. Not Use of FISH to detect eukaryotic picoplankton
• I. Biegala Use of FISH in detecting bacteria associated with dinoflagellates

09.00-09.25 D. Vaulot & S.Y. Moon Diversity of eukaryotic picoplankton in the Pacific

09.25-09.50 W.H.C.F. Kooistra, and R.P.T. Koeman Detection of Raphidophyceae, potentially harmful microalgae

09.50-10.15 A.C. Barbrook and C.J. Howe Minicircular plastid DNA markers in dinoflagellates.

10.15-10.45

1045-1115 Coffee Break

1115-1300 Roundtable discussion on problems etc associated with environmental gene cloning

1115-1130 A Wilmotte MICROMAT: Workshop on DNA extraction methods

Last updated 13 July 2000